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KMID : 1234420080360020158
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Korean Journal of Microbiololgy and Biotechnology
2008 Volume.36 No. 2 p.158 ~ p.164
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Breeding of Yeast Strain with Starch Utilizing and Alcohol Fermenting Ability by Protoplast Fusion
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Ju Min-No
Hong Sung-Wook
Kim Kwan-Tae
Yum Sung-Kwan
Kim Gye-Won
Chung Kun-Sub
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Abstract
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The fusants which contain starch utilizing ability and alcohol fermenting ability were developed by protoplast fusion of Saccharomyces cerevisiae KOY-1 and Saccharomyces diastaticus KCTC 1804. Saccharomyces cerevisiae KH-12 was obtained by haploid induction from Saccharomyces cerevisiae KOY-1. The auxotropic mutants of yeast were obtained by using an ethylmethane sulfonate (EMS). The frequency of protoplast formation in Saccharomyces cerevisiae KOY-1 (Met?) and Saccharomyces diastaticus KCTC 1804 (Trp?) were 90.5% and 97.7%, respectively. The frequency of fusant formation was 1.79 × 10-4 for the regenerated protoplast and the 1,000 fusants were obtained. Fusant FA 776 was selected as a potential yeast which contain an alcohol fermenting ability in the starch medium. The genetic stability was 4.64% for 10 passages of generation. Fusant FA 776 produced 13 mg/ml of alcohol in 24% starch medium and showed 1.86-fold higher alcohol fermenting ability than Saccharomyces diastaticus KCTC 1804.
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KEYWORD
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Starch utilizing, alcohol fermenting, protoplast fusion
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